Studies on l-tyrosine O-sulphate. 3. The metabolic fate of the L-tyrosine O[35S]-sulphate residue of 35S-labelled rabbit fibrinopeptide B

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Biosynthesis of L-tyrosine O-sulphate from the Methyl and Ethyl Esters of L-tyrosine.

1. Rat-liver supernatant preparations are capable of achieving the biological sulphation of l-tyrosine methyl ester, the reaction proceeding maximally at a substrate concentration of 30 mm and at pH 7.0. 2. Two sulphated products are formed, one of which has been identified as l-tyrosine O-sulphate. On the basis of indirect evidence the other product can be assumed to be l-tyrosine O-sulphate m...

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A rat-tissue aminotransferase acting on L-tyrosine O-sulphate.

1. Rat tissues have been shown to possess an aminotransferase that is active towards l-tyrosine O-sulphate and dependent on 2-oxoglutarate and pyridoxal phosphate. 2. Kidney, liver and pancreas have the greatest activity and the enzyme is localized mainly in the mitochondrial fraction in the liver and kidney cell. 3. The enzyme was shown to be distinct from l-tyrosine-2-oxoglutarate aminotransf...

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The detection and determination of L-tyrosine O-sulphate in rabbit and other mammalian urine.

A sensitive method using the Technicon Amino Acid Autoanalyzer was developed for the determination of l-tyrosine O-sulphate in mammalian urine. Normal rabbit urine was shown to contain a material that has properties identical with l-tyrosine O-sulphate. The material is also a normal constituent of rat, sheep, calf and mouse urine and is excreted by these animals in quantities ranging from 0.1 t...

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Studies on L - Tyrosine O - Sulphate 2 . IDENTIFICATION OF p - HYDROXYPHENYLPYRUVIC ACID [ 35 S ] SULPHATE AS A METABOLITE OF L - TYROSINE O [ 35 S ] - SULPHATE IN THE RAT BY GILLIAN

Cavallini, D. & Stirpe, F. (1957). Ital. J. Biochem. 6, 1. Dodgson, K. S., Powell, G. M., Rose, F. A. & Tudball, N. (1961). Biochem. J. 79, 209. Dodgson, K. S., Powell, G. M. & Tudball, N. (1960). Biochem. biophy8. Be8. Commun. 2, 130. Dodgson, K. S. & Tudball, N. (1960). Biochem. J. 74, 154. Doy, C. H. (1960). Nature, Lond., 529, 4724. Knox, W. E. & Pitt, B. M. (1957). J. biol. Chem. 225, 675....

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Tyrosine O-sulphate in Fibrinogen and Fibrin.

This paper describes the details of the identification and determination of tyrosine 0-sulphate in fibrinogen, fibrin and fibrinopeptide B, as already reported in preliminary communications by the author under his previous names (Bettelheim, 1954; Bettelheim-Jevons, 1958). The first indication of the presence of tyrosine in modified form came during work on the peptides liberated from bovine fi...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1963

ISSN: 0006-2936

DOI: 10.1042/bj0870548